Anti-Kif2a抗体(ab71160)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to Kif2a
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish, Platypus
  • 免疫原

    Synthetic peptide corresponding to a region between residue 630 and 679 of human Kif2a (NP_004511.1)

  • 阳性对照
    • Whole cell lysates from Hela cells, 293T cells and NIH3T3 cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明
    IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 80 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity.
    • 序列相似性
      Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.
      Contains 1 kinesin-motor domain.
    • 细胞定位
      Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle pole. Cytoplasm > cytoskeleton > spindle. Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CDCBM3 antibody
      • HK2 antibody
      • KIF2 antibody
      • KIF2A antibody
      • KIF2A_HUMAN antibody
      • Kinesin 2 antibody
      • Kinesin heavy chain 2 antibody
      • Kinesin heavy chain member 2 antibody
      • Kinesin heavy chain member 2A antibody
      • Kinesin like protein KIF2A antibody
      • Kinesin-2 antibody
      • Kinesin-like protein KIF2A antibody
      • KNS2 antibody
      • M Kinesin antibody
      see all

    图片

    • All lanes : Anti-Kif2a antibody (ab71160) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


      Predicted band size : 80 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human Kif2a by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab71160 at 3 µg/mg lysate for IP (Lane 1) and at 0.1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

    文献

    ab71160 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Xenopus laevis Cell lysate - whole cell (Xenopus laevis cytoplasmic egg extract)
    Loading amount
    40 µg
    Specification
    Xenopus laevis cytoplasmic egg extract
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 04 2011

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Xenopus laevis Cell (Xenopus laevis cytoplasmic egg extract)
    Specification
    Xenopus laevis cytoplasmic egg extract
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 04 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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