RabMAb

Anti-KIF22抗体[EP2747Y] (ab75783)

概述

  • 产品名称
    Anti-KIF22抗体[EP2747Y]
    参阅全部 KIF22 一抗
  • 描述
    兔单克隆抗体[EP2747Y] to KIF22
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues on the N-terminus of KIF22

  • 阳性对照
    • HeLa cell lysate
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).
IP 1/10.
Flow Cyt 1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.
  • 序列相似性
    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • 翻译后修饰
    Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A 328A3.2 antibody
    • KID antibody
    • KIF 22 antibody
    • kif22 antibody
    • KIF22_HUMAN antibody
    • Kinesin family member 22 antibody
    • Kinesin like 4 antibody
    • Kinesin like DNA binding protein antibody
    • Kinesin like DNA binding protein pseudogene antibody
    • Kinesin like protein 4 antibody
    • Kinesin like protein KIF22 antibody
    • Kinesin-like DNA-binding protein antibody
    • Kinesin-like protein 4 antibody
    • Kinesin-like protein KIF22 antibody
    • KNSL 4 antibody
    • KNSL4 antibody
    • OBP 1 antibody
    • OBP 2 antibody
    • OBP antibody
    • OBP1 antibody
    • OBP2 antibody
    • Origin of plasmid DNA replication binding protein antibody
    • OriP binding protein antibody
    • OTTHUMP00000123406 antibody
    • SEMDJL2 antibody
    see all

图片

  • Anti-KIF22 antibody [EP2747Y] (ab75783) at 1/5000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled oat anti rabbit at 1/2000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

文献

This product has been referenced in:
  • Li C  et al. NuSAP governs chromosome oscillation by facilitating the Kid-generated polar ejection force. Nat Commun 7:10597 (2016). IF . Read more (PubMed: 26839278) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - nuclear (cartilage)
Loading amount
10 µg
Specification
cartilage
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 26 2012

Thank you for contacting us and for your enquiry. As far as we are aware, non of our KIF22 antibodies has been tested with samples from zebrafish. All tested and guaranteed species cross-reactivity information is stated on our datasheets, and these...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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