antigen: RKTQEVSALR RLAKPMSERV AGRAGLKPPM LDSGAEVSAS TTSSEAESGA RSVSSIVRQW NRKINHFLGD HPAPTVNGTR PARKKFQKKG, corresponding to amino acids 814-903 of Human KIF21B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Microtubule-binding motor protein probably involved in neuronal dendritic transport. In vitro, has a plus-end directed motor activity.
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Contains 7 WD repeats.
Cytoplasm > cytoskeleton.
Information by UniProt
- KI21B_HUMAN antibody
- KIAA0449 antibody
- KIF21B antibody
- Kinesin-like protein KIF21B antibody
Immunocytochemistry/ Immunofluorescence - Anti-KIF21B antibody (ab121931)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121931 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF21B antibody (ab121931)
ab121931 at 1/125 dilution, staining KIF21B in paraffin embedded Human kidney tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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