概述

  • 产品名称Anti-KIF20A抗体
    参阅全部 KIF20A 一抗
  • 描述
    兔多克隆抗体to KIF20A
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence

    KRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRR

    , amino acids 828-877 of Human KIF20A (NP_005724)

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab85644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 100 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.
  • 序列相似性Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • 翻译后修饰Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.
  • 细胞定位Golgi apparatus. Cytoplasm > cytoskeleton > spindle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA415432 antibody
    • FLJ21151 antibody
    • GG10 2 antibody
    • GG10_2 antibody
    • KI20A_HUMAN antibody
    • KIF20A antibody
    • kinesin family member 20A antibody
    • Kinesin like protein KIF20A antibody
    • Kinesin-like protein 174 antibody
    • Kinesin-like protein KIF20A antibody
    • mitotic kinesin like protein 2 antibody
    • Mitotic kinesin-like protein 2 antibody
    • MKlp2 antibody
    • OTTHUMP00000159481 antibody
    • RAB6 interacting, kinesin like (rabkinesin6) antibody
    • Rab6-interacting kinesin-like protein antibody
    • RAB6-INTERACTING PROTEIN, KINESIN-LIKE antibody
    • RAB6KIFL antibody
    • Rabkinesin 6 antibody
    • Rabkinesin-6 antibody
    see all

Anti-KIF20A antibody 图像

  • Anti-KIF20A antibody (ab85644) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 100 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
  • ab85644 staining KIF20A in spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab85644 staining KIF20A in thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-KIF20A antibody (ab85644)参考文献

ab85644 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85644.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"