产品名称 Anti-KIF1C抗体 参阅全部 KIF1C 一抗
ICC/IF, WB more details
Recombinant fragment, corresponding to a region within amino acids 452-718 of Human KIF1C (O43896).
A549, HepG2 and HCT116 whole cell lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
存储溶液 pH: 7.00 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab125902 is purified by antigen-affinity chromatography.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/3000. Predicted molecular weight: 123 kDa.
功能 Motor required for the retrograde transport of Golgi vesicles to the endoplasmic reticulum. Has a microtubule plus end-directed motility.
组织特异性 Expressed in all tissues examined, with most abundant expression in heart and skeletal muscle.
疾病相关 Spastic ataxia 2, autosomal recessive
序列相似性 Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Unc-104 subfamily. Contains 1 FHA domain. Contains 1 kinesin motor domain.
翻译后修饰 Phosphorylated on tyrosine residues.
细胞定位 Cytoplasm, cytoskeleton.
Information by UniProt
Kinesin family member 1C antibody
Anti-KIF1C antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-KIF1C antibody (ab125903) This image is courtesy of an anonymous Abreview
ab125903 staining KIF1C in Human skin fibroblast cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% BSA and 0.1% Triton) for 18 hours at 4°C. An Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
Western blot - Anti-KIF1C antibody (ab125903)
Anti-KIF1C antibody (ab125903) at 1/500 dilution + HepG2 whole cell lysate at 30 µg Predicted band size : 123 kDa
5% SDS PAGE
Secondary antibody -
anti-rabbit HRP ( ab6721)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"