概述

  • 产品名称Anti-KIF1C抗体
    参阅全部 KIF1C 一抗
  • 描述
    兔多克隆抗体to KIF1C
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 452-718 of Human KIF1C (O43896).

  • 阳性对照
    • A549, HepG2 and HCT116 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab125903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/3000. Predicted molecular weight: 123 kDa.

靶标

Anti-KIF1C antibody 图像

  • ab125903 staining KIF1C in Human skin fibroblast cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 5% BSA and 0.1% Triton) for 18 hours at 4°C. An Cy2®-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-KIF1C antibody (ab125903) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 123 kDa

    5% SDS PAGE

     

    Secondary antibody - anti-rabbit HRP (ab6721)

Anti-KIF1C antibody (ab125903)参考文献

This product has been referenced in:
  • Dor T  et al. KIF1C mutations in two families with hereditary spastic paraparesis and cerebellar dysfunction. J Med Genet 51:137-42 (2014). WB ; Human . Read more (PubMed: 24319291) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (Skin Fibroblasts)
Specification Skin Fibroblasts
Permeabilization Yes - 0.1% triton
Fixative Methanol
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Verified customer

提交于 Jul 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Skin Fibroblasts)
Loading amount 30 µg
Specification Skin Fibroblasts
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"