Anti-KIF1A抗体(ab91029)

概述

  • 产品名称Anti-KIF1A抗体
    参阅全部 KIF1A 一抗
  • 描述
    兔多克隆抗体to KIF1A
  • 经测试应用适用于: IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1500 - 1600 of Human KIF1A.

    (Peptide available as ab108241.)

  • 阳性对照
    • This antibody gave a positive signal in both Mouse brain embryonic E14 and Mouse brain embryonic E18 tissue lysates. As well as human hippocampus formalin fixed paraffin embedded tissue section within immunohistochemistry.

性能

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应用

Our Abpromise guarantee covers the use of ab91029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).
ELISA Use a concentration of 1 µg/ml.

靶标

  • 功能Motor for anterograde axonal transport of synaptic vesicle precursors.
  • 序列相似性Belongs to the kinesin-like protein family. Unc-104 subfamily.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
    Contains 1 PH domain.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ATSV antibody
    • Axonal transporter of synaptic vesicles antibody
    • C2orf20 antibody
    • HSN2C antibody
    • hUnc 104 antibody
    • hUnc-104 antibody
    • hunc104 antibody
    • Kif1a antibody
    • KIF1A related protein antibody
    • KIF1A_HUMAN antibody
    • Kinesin family member 1A antibody
    • Kinesin heavy chain member 1A homolog of mouse antibody
    • kinesin like protein antibody
    • kinesin like protein KIF1A antibody
    • Kinesin-like protein KIF1A antibody
    • Microtubule based motor antibody
    • Microtubule based motor KIF1A antibody
    • Microtubule-based motor KIF1A antibody
    • MRD9 antibody
    • SPG30 antibody
    • Unc 104 and KIF1A related protein antibody
    • Unc 104 antibody
    • Unc-104- and KIF1A-related protein antibody
    • UNC104 antibody
    see all

Anti-KIF1A antibody 图像

  • All lanes : Anti-KIF1A antibody (ab91029) at 1 µg/ml

    Lane 1 : E14 Mouse Embryo Brain Tissue Lysate
    Lane 2 : E18 Ms Embryo Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 191 kDa
    Observed band size : 191 kDa
    Additional bands at : 58 kDa,95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of KIF1A staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91029, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-KIF1A antibody (ab91029)参考文献

ab91029 has not yet been referenced specifically in any publications.

Product Wall


The exact immunogen is proprietary information.

I checked the homology between human Kif1A and B and it is for the entire protein between 64 and 77%.

However, for the specific immunogen region (within residues 1500 - 1600 of Huma...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"