Anti-KIF15抗体(ab90735)

概述

  • 产品名称Anti-KIF15抗体
    参阅全部 KIF15 一抗
  • 描述
    兔多克隆抗体to KIF15
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    A synthetic peptide corresponding to a region within internal sequence amino acids 1080 - 1129 (

    KKHSGLLQSAQEELTKKEALIQELQHKLNQKKEEVEQKKNEYNFKMRQLE

    ) of Human KIF15 (NP_064627)

  • 阳性对照
    • COLO205 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab90735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 160 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性KIF15 belongs to the kinesin-like protein family and contains 1 kinesin-motor domain. KIF15 is the plus-end directed kinesin-like motor enzyme involved in mitotic spindle assembly.
  • 细胞定位Cytoplasmic, Cytoskeleton, Microtubule Note: Detected during the interphase in the cytoplasm as finely punctuate pattern and irregularly shaped dots. Detected during mitosis on the mitotic spindle. Colocalizes with TPX2 in mitosis. Localizes at the central spindle at anaphase.
  • 数据库链接
  • 别名
    • FLJ25667 antibody
    • Kinesin like protein 7 antibody
    • KLP2 antibody
    • HKLP2 antibody
    • Kinesin family member 15 antibody
    • Kinesin like protein 2 antibody
    • Kinesin like protein KIF15 antibody
    • KNSL7 antibody
    • Linesin like 7 antibody
    • NY BR 62 antibody
    • NYBR62 antibody
    • Serologically defined breast cancer antigen NY BR 62 antibody
    • Serologically defined breast cancer antigen NYBR62 antibody
    see all

Anti-KIF15 antibody 图像

  • Anti-KIF15 antibody (ab90735) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 160 kDa

Anti-KIF15 antibody (ab90735)参考文献

ab90735 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Oct 27 2015

Thanks for your reply.

I received a reply from my lab colleagues who informed that the intense band at 65-70kD is either a degradation product of the full-length intact protein, or a non-specific band altogether. We are not absolutely sure ex...

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Thank you for your reply.

Although we don’t normally offer discount codes for two different untested species / applications, I am happy to make an exception in this occasion as the antibody shares high homology with the mouse protein. <...

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Thank you for contacting us.

Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab90735.

Unfortunately ab90735 has not be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"