概述

  • 产品名称Anti-KIF14抗体
    参阅全部 KIF14 一抗
  • 描述
    兔多克隆抗体to KIF14
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14) encoded within exon 16.

  • 阳性对照
    • HeLa whole cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab3747 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/250 - 1/1000.

靶标

  • 功能Plays an essential role in cytokinesis.
  • 序列相似性Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • 发展阶段Up-regulated in cells progressing through G2/M phase.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Anti-KIF14 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling KIF14 with ab3747 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 190 kDa

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

    Sample: HeLa whole cell lysate

    1) WB - 50µg

    2) IP - 1mg

    Antibody: Affinity purified Rabbit anti-KIF (ab3747)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

    Detection: ECL.

  • Whole cell lysate (50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. ab3747 used for western blot at 1 µg/ml and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lane 1 - Input.

    Lane 2 - IP.

    Lane 3 - Control IgG IP.

Anti-KIF14 antibody (ab3747)参考文献

ab3747 has not yet been referenced specifically in any publications.

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