概述

  • 产品名称Anti-KIF14抗体
    参阅全部 KIF14 一抗
  • 描述
    兔多克隆抗体to KIF14
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Kinesin Family Member 14 (KIF 14)encoded within exon 2.

  • 阳性对照
    • HeLa whole cell extract.

性能

应用

Our Abpromise guarantee covers the use of ab3746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution. PubMed: 16648480
WB 1/2000 - 1/10000. Predicted molecular weight: 190 kDa.
IP Use a concentration of 1 - 4 µg/ml.

靶标

  • 功能Plays an essential role in cytokinesis.
  • 序列相似性Belongs to the kinesin-like protein family.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • 发展阶段Up-regulated in cells progressing through G2/M phase.
  • 细胞定位Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > spindle. Nuclear localization observed during interphase in PubMed:16431929 or triggered by entry into mitosis in PubMed:16648480. Cytoplasmic in interphase (PubMed:16648480) and metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0042 antibody
    • KIF 14 antibody
    • KIF14 antibody
    • KIF14_HUMAN antibody
    • Kinesin Family Member 14 antibody
    • Kinesin like protein KIF14 antibody
    • Kinesin-like protein KIF14 antibody
    • MGC142302 antibody
    see all

Anti-KIF14 antibody 图像

  • Sample: Hela whole cell lysate

    1) WB - 50µg.

    2) IP - 1mg.

     

    Antibody: Affinity purified Rabbit anti-KIF (ab3746)

    1) & 2) WB - 1 / 4000 dilution.

    2) IP - concentration of 3µg / mg of lysate.

     

    Detection: ECL.

     

  • ICC/IF image of ab3746 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3746, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-KIF14 antibody (ab3746)参考文献

This product has been referenced in:
  • Carleton M  et al. RNA interference-mediated silencing of mitotic kinesin KIF14 disrupts cell cycle progression and induces cytokinesis failure. Mol Cell Biol 26:3853-63 (2006). ICC/IF ; Human . Read more (PubMed: 16648480) »

See 1 Publication for this product

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