Anti-KIF13B抗体(ab86528)

概述

  • 产品名称Anti-KIF13B抗体
    参阅全部 KIF13B 一抗
  • 描述
    兔多克隆抗体to KIF13B
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 108-157 (GKSYTMMGTA DQPGLIPRLC SGLFERTQKE ENEEQSFKVE VSYMEIYNEK) of Human KIF13B (NP_056069).

  • 阳性对照
    • HepG2 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab86528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 203 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May be involved in reorganization of the cortical cytoskeleton. May be functionally important for the intracellular trafficking of MAGUKs and associated protein complexes.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the kinesin-like protein family.
    Contains 1 CAP-Gly domain.
    Contains 1 FHA domain.
    Contains 1 kinesin-motor domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GAKIN antibody
    • Guanylate kinase associated kinesin antibody
    • KI13B_HUMAN antibody
    • KIAA0639 antibody
    • KIF 13B antibody
    • KIF13B antibody
    • Kinesin 13B antibody
    • Kinesin family member 13B antibody
    • Kinesin like protein GAKIN antibody
    • Kinesin like protein KIF13B antibody
    • Kinesin-like protein GAKIN antibody
    • Kinesin-like protein KIF13B antibody
    • OTTHUMP00000225649 antibody
    see all

Anti-KIF13B antibody 图像

  • IHC image of KIF13B staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86528, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-KIF13B antibody (ab86528) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 203 kDa
    Observed band size : 203 kDa
    Additional bands at : 38 kDa,65 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 6-18%
  • Anti-KIF13B antibody (ab86528) at 1 µg/ml (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of human AGS Gastric carcinoma cells at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 203 kDa
    Observed band size : 200 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% BSA for 1 hour at 23°C

    See Abreview

Anti-KIF13B antibody (ab86528)参考文献

ab86528 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 Mouse Fibroblasts)
Loading amount 10 µg
Specification NIH 3T3 Mouse Fibroblasts
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount 20 µg
Specification AGS Gastric Carcinoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Jun 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"