概述

  • 产品名称Anti-KIF12抗体
    参阅全部 KIF12 一抗
  • 描述
    兔多克隆抗体to KIF12
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: LLSLGSPRPL PVRWNKTRGF YVEQLRVVEF GSLEALMELL QTGLSRRRNS , corresponding to N terminal amino acids 36 - 85 of Human KIF12 (NP_612433)

  • 阳性对照
    • 721 B cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab80448 is purified by a peptide affinity chromatography method
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 71 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

Anti-KIF12 antibody 图像

  • Anti-KIF12 antibody (ab80448) at 1 µg/ml + 721 B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 - 1/100000

    Predicted band size : 71 kDa
    Observed band size : 56 kDa (why is the actual band size different from the predicted?)

Anti-KIF12 antibody (ab80448)参考文献

This product has been referenced in:
  • Tan MH  et al. Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer. Breast Cancer Res Treat 131:849-58 (2012). WB ; Human . Read more (PubMed: 21479552) »

See 1 Publication for this product

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