概述

  • 产品名称Anti-KIBRA抗体
    参阅全部 KIBRA 一抗
  • 描述
    兔多克隆抗体to KIBRA
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 289-327 of Human KIBRA (NP_056053.1).

  • 阳性对照
    • ZR-75-1 cell line lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human Kidney.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab107637 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab107637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 125 kDa.

靶标

  • 相关性KIBRA is a WW domain containing protein, first identified by yeast 2-hybrid analysis of a human brain cDNA expression library. Gene expression studies have shown that KIBRA is expressed in memory-related brain structures including the hippocampus (dentate gyrus and the CA1 region) and the temporal lobe, 2 key regions for memory, and that the expression of specific KIBRA alleles is significantly associated with memory performance.
  • 细胞定位Cytoplasm. Cytoplasm › perinuclear region. Nucleus. Cell projection › ruffle membrane. Note: Co-localizes with PRKCZ in the perinuclear region.
  • 数据库链接
  • 别名
    • FLJ10865 antibody
    • FLJ23369 antibody
    • HBeAg-binding protein 3 antibody
    • HBEBP3 antibody
    • HBEBP36 antibody
    • KIAA0869 antibody
    • KIBRA antibody
    • Kidney and brain protein antibody
    • Protein KIBRA antibody
    • Protein WWC1 antibody
    • WW and C2 domain containing 1 antibody
    • WW C2 and coiled-coil domain containing 1 antibody
    • WW domain-containing protein 1 antibody
    • WWC1 antibody
    see all

Anti-KIBRA antibody 图像

  • IHC image of KIBRA staining in Human Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107637, 10 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-KIBRA antibody (ab107637) at 1/100 dilution + ZR-75-1 cell line lysate at 35 µg

    Predicted band size : 125 kDa

Anti-KIBRA antibody (ab107637)参考文献

This product has been referenced in:
  • Moleirinho S  et al. KIBRA exhibits MST-independent functional regulation of the Hippo signaling pathway in mammals. Oncogene : (2012). Human . Read more (PubMed: 22614006) »

See 1 Publication for this product

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Thank you for contacting us.

This antibody has not been tested in IHC and does not have any publications for this application.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"