Anti-KIAA1967抗体(ab70239)

概述

  • 产品名称Anti-KIAA1967抗体
    参阅全部 KIAA1967 一抗
  • 描述
    兔多克隆抗体to KIAA1967
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of Human KIAA1967 (UniProt: Q8N163).

  • 阳性对照
    • Nuclear extract from HeLa cells

性能

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应用

Our Abpromise guarantee covers the use of ab70239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
IP Use at 1-10 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.
  • 组织特异性Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCAR2 antibody
    • Cell cycle and apoptosis regulator 2 antibody
    • Cell division cycle and apoptosis regulator protein 2 antibody
    • DBC-1 antibody
    • DBC.1 antibody
    • DBC1 antibody
    • DBIRD complex subunit KIAA1967 antibody
    • Deleted in breast cancer 1 antibody
    • Deleted in breast cancer gene 1 protein antibody
    • K1967_HUMAN antibody
    • KIAA1967 antibody
    • NET35 antibody
    • p30 DBC antibody
    • p30 DBC protein antibody
    • p30DBC antibody
    see all

Anti-KIAA1967 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling KIAA1967 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-KIAA1967 antibody (ab70239) at 1/10000 dilution

    Lane 1 : HeLa cell nuclear extract at 15 µg
    Lane 2 : HeLa cell nuclear extract at 45 µg


    Predicted band size : 103 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)

Anti-KIAA1967 antibody (ab70239)参考文献

ab70239 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK)
Loading amount 50 µg
Specification HEK
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Apr 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"