The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 5-15µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 121 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
KIAA1688 was identified as a hypothetical protein predicted from the in silico analysis of long cDNAS isolated in the Kazusa cDNA sequencing project. The function of KIAA1688 has not been characterized.
All lanes : Anti-KIAA1688 antibody (ab93780) at 0.1 µg/ml
Lane 1 : HeLa cell lysate at 50 µg Lane 2 : HeLa cell lysate at 15 µg Lane 3 : HeLa cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 121 kDa
Exposure time : 3 minutes
Immunoprecipitation - KIAA1688 antibody (ab93780)
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab93780 at 10 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot : KIAA1688 antibody (ab93780) at 1 µg/ml
Developed using the ECL technique Exposure time : 3 minutes