antigen sequence: DVEVENEVKE GRGHTPEGTL FLREDAKPPD LELKRVSLGP QWQPGPGLGS HQPHPLDSRT PCRTAYATTA PMTPESSGPG, corresponding to amino acids 682-761 of Human KIAA1614.
- Human adrenal gland tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
- K1614_HUMAN antibody
- KIAA1614 antibody
- RP11-46A10.3 antibody
- Uncharacterized protein KIAA1614 antibody
Immunocytochemistry/ Immunofluorescence - Anti-KIAA1614 antibody (ab121978)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121978 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA1614 antibody (ab121978)
ab121978 at 1/10 dilution staining KIAA1614 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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