概述

  • 产品名称Anti-KIAA1045抗体
    参阅全部 KIAA1045 一抗
  • 描述
    兔多克隆抗体to KIAA1045
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    antigen sequence: LLTEEEMYSL TETFQRCKVI PDCSLTLEDF LRYRHQAAKR GDRDRALSEE QEEQAARQFA ALDPEHRGHI EWPDFLSHES LLLLQQLR, corresponding to amino acids 194-281 of Human KIAA1045.

  • 阳性对照
    • RT4, U 251 MG, Human Liver and Human Tonsil lysates; Human pancreas tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-KIAA1045 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121643 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-KIAA1045 antibody (ab121643) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
    10-20% SDS PAGE
  • ab121643 at 1/500 dilution staining KIAA1045 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.

Anti-KIAA1045 antibody (ab121643)参考文献

ab121643 has not yet been referenced specifically in any publications.

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