Anti-KIAA0513抗体(ab121430)
Key features and details
- Rabbit polyclonal to KIAA0513
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-KIAA0513抗体 -
描述
兔多克隆抗体to KIAA0513 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
N terminal amino acids 7-123 of Human KIAA0513.
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阳性对照
- Human skeletal muscle tissue; RT-4 and Human liver tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121430于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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组织特异性
Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9764 Human
- Omim: 611675 Human
- SwissProt: O60268 Human
- Unigene: 301658 Human
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别名
- K0513_HUMAN antibody
- KIAA0513 antibody
- Uncharacterized protein KIAA0513 antibody
图片
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All lanes : Anti-KIAA0513 antibody (ab121430) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human plasma
Lanes 4-5 : Human Liver tissue lysate
Developed using the ECL technique. -
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab121430 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121430, at a 1/45 dilution, staining KIAA0513 in paraffin-embedded Human skeletal muscle, by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121430 尚未被引用在任何文献中。