Chimpanzee, Gorilla, Orangutan
, corresponding to amino acids 1000-1050 of Human KIAA0460.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Antibody was affinity purified using an epitope specific to KIAA0460 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 155 kDa).
||Use at an assay dependent dilution.
This is a hypothetical protein fragment believed to be involved in DNA repair.
KIAA0460 contains 1 CID domain.
- FLJ 32145 antibody
- HSPC 099 antibody
- HSPC099 antibody
Western blot - Anti-KIAA0460 antibody (ab17996)
Lanes 1-2 :
Anti-KIAA0460 antibody (ab17996) at 1 µg/mlLanes 3-4 :
Anti-KIAA0460 antibody (ab17996) at 0.4 µg/mlLanes 1 & 3 :
Whole cell lysate from HEK239 cells at 15 µgLanes 2 & 4 :
Whole cell lysate from HEK239 cells at 50 µgPredicted band size:
155 kDaObserved band size:
225 kDa (why is the actual band size different from the predicted?
Detection of Human KIAA0460 by Western Blot.
Samples: Whole cell lysate (15 and 50 µg) from HEK293 cells.
Antibody: Affinity purified goat anti-KIAA0460 antibody used at 1 and 0.4 mg/ml.
Detection: Chemiluminescence with an exposure time of 1 minute.
has not yet been referenced specifically in any publications.
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