Anti-KIAA0427抗体(ab82891)

概述

  • 产品名称Anti-KIAA0427抗体
    参阅全部 KIAA0427 一抗
  • 描述
    兔多克隆抗体to KIAA0427
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (QVQGLLADKT EGDGESERTQ SHISQWTADC SEPLDSSCSF SRGRAPPQQN) of Human KIAA0427 (NP_055587).

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/12500.

靶标

  • 功能Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the CTIF family.
    Contains 1 MIF4G domain.
  • 细胞定位Cytoplasm > perinuclear region.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CBP80/20-dependent translation initiation factor antibody
    • ctif antibody
    • CTIF_HUMAN antibody
    • Gm672 antibody
    • Hypothetical protein LOC9811 antibody
    • Uncharacterized protein KIAA0427 antibody
    see all

Anti-KIAA0427 antibody 图像

  • Anti-KIAA0427 antibody (ab82891) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

Anti-KIAA0427 antibody (ab82891)参考文献

ab82891 has not yet been referenced specifically in any publications.

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