Anti-KIAA0319抗体(ab118923)

概述

  • 产品名称
    Anti-KIAA0319抗体
    参阅全部 KIAA0319 一抗
  • 描述
    兔多克隆抗体to KIAA0319
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 151-200 (EEMSEYSDDY RELEKDLLQP SGKQEPRGSA EYTDWGLLPG SEGAFNSSVG) of Human KIAA0319 (EAW55455).

  • 阳性对照
    • HepG2 cell lysate; Human kidney tissue

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Constituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能
    Involved in neuronal migration during development of the cerebral neocortex. May function in a cell autonomous and a non-cell autonomous manner and play a role in appropriate adhesion between migrating neurons and radial glial fibers. May also regulate growth and differentiation of dendrites.
  • 组织特异性
    Detected in adult brain cortex and fetal frontal lobe (at protein level). Highly expressed in brain cortex, putamen, amygdala, hippocampus and cerebellum.
  • 疾病相关
    Defects in KIAA0319 may be a cause of susceptibility to dyslexia type 2 (DYX2) [MIM:600202]; also known as specific reading disability type 2. Dyslexia is a relatively common, complex cognitive disorder that affects 5% to 10% of school-aged children. The disorder is characterized by an impairment of reading performance despite adequate motivational, educational and intellectual opportunities and in the absence of sensory or neurological disability. Note=A lower expression is associated with the risk haplotype.
  • 序列相似性
    Contains 1 MANSC domain.
    Contains 5 PKD domains.
  • 发展阶段
    Expressed in the developing cerebral neocortex and glanglionic eminence in 57 days post-fertilization fetal brain.
  • 翻译后修饰
    N-glycosylated.
    O-glycosylated.
    Shedding of the extracellular domain and intramembrane cleavage produce several proteolytic products. The intramembrane cleavage releases a soluble cytoplasmic polypeptide that translocates to the nucleolus.
  • 细胞定位
    Cell membrane. Early endosome membrane. Low-abundance isoforms lacking the transmembrane domain have been described; these are secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DLX 2 antibody
    • DLX2 antibody
    • DYLX 2 antibody
    • DYLX2 antibody
    • Dyslexia susceptibility 2 antibody
    • Dyslexia-associated protein KIAA0319 antibody
    • DYX 2 antibody
    • DYX2 antibody
    • K0319_HUMAN antibody
    • Kiaa0319 antibody
    • MGC176717 antibody
    see all

图片

  • Anti-KIAA0319 antibody (ab118923) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 56 kDa
  • ab118923, at 8 µg/ml, staining KIAA0319 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    Cells with positive label are epithelial cells of renal tubules (indicated by arrows).

文献

ab118923 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-10 % gradient gel)
Loading amount
100 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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提交于 Jul 21 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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