The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
Use neat. Note: samples are acetone fixed.
功能Thought to be required for maintaining cell proliferation.
序列相似性Contains 1 FHA domain.
发展阶段Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
细胞定位Nucleus. Nucleus, nucleolus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.