Anti-Ki67抗体- BSA and Azide free (ab80827)

概述

  • 产品名称Anti-Ki67抗体- BSA and Azide free
    参阅全部 Ki67 一抗
  • 描述
    兔多克隆抗体to Ki67 - BSA and Azide free
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Ki67 (C terminal).

  • 阳性对照
    • Tonsil; breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab80827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: Use at a concentration of 10 - 20 µg/ml for 30 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

    This antibody is excellent for staining of formalin/paraffin tissues and serves as a marker of growth fraction.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.
    • 序列相似性Contains 1 FHA domain.
      Contains 16 K167R repeats.
      Contains 1 PP1-binding domain.
    • 发展阶段Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
    • 翻译后修饰Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
    • 细胞定位Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antigen identified by monoclonal antibody Ki 67 antibody
      • Antigen identified by monoclonal antibody Ki-67 antibody
      • Antigen KI-67 antibody
      • Antigen KI67 antibody
      • Antigen Ki67 antibody
      • KI67_HUMAN antibody
      • KIA antibody
      • Marker of proliferation Ki-67 antibody
      • MIB 1 antibody
      • MIB antibody
      • MKI67 antibody
      • PPP1R105 antibody
      • Proliferation marker protein Ki-67 antibody
      • Proliferation related Ki 67 antigen antibody
      • Protein phosphatase 1 regulatory subunit 105 antibody
      • RP11-380J17.2 antibody
      see all

    Anti-Ki67 antibody - BSA and Azide free 图像

    • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human tonsil tissue stained with ab80827 at 20µg/ml, in conjunction with a peroxidase-conjugated secondary antibody and an AEC chromogen. Note nuclear staining of proliferating cells.

    Anti-Ki67 antibody - BSA and Azide free (ab80827)参考文献

    ab80827 has not yet been referenced specifically in any publications.

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