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Our Abpromise guarantee covers the use of ab833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/400. PubMed: 18635871|
|ICC||Use at an assay dependent concentration.|
|IHC-P||1/25 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. We recommend using an ABC system for detection of this antibody.|
|IHC-Fr||1/25 - 1/50. 1/25 - 1/50. We suggest an incubation period of 30 minutes at room temperature and to use DAB to stain the protein.|
ab833 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with Ki-67, using ABC and AEC chromogen.
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