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Our Abpromise guarantee covers the use of ab66155 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 19520159|
|IHC-Fr||Use a concentration of 1.6 µg/ml.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20729478|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 359 kDa.|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formaldehyde fixed mouse skin sections labelling Ki67 with ab66155 at a dilution of 1:1000. The secondary antibody used was a goat anti-rabbit IgG (HRP). Antigen retrieval was performed using Citrate buffer pH 6.0 for 20 minutes at 97ºC.
ab66155 staining Ki67 in human LnCAP cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with ethanol and triton, and blocked for 1 hour at 26°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 16 hours at 4°C. An undiluted IRDye® 800CW-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.
ab66155 staining Ki67 in murine brain tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/200 dilution for 12 hours at 4°C. ab96919 Donkey polyclonal to anti-rabbit DyLight® 488 (IgG - H&L), pre-adsorbed was used as the secondary antibody undiluted.
ab66155 staining ki67 in proliferating mouse astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.3% TritonX 100 and 3M Glycine and blocked with 10% BSA for 20 minutes at 20°C. Samples were incubated with primary antibody (1/250 in 4% BSA/PBS) for 18 hours at 4°C. ab150073 at a dilution of 1/500 was used as the secondary antibody.
Astrocytes (orange) labelled with Ki67 proliferation marker (violet) and counter stained with DAPI (blue).
Scale bar 20um, magnification x40 oil.
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