The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 mg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Detected in neural and non-neural cell lines.
Belongs to the KHSRP family. Contains 4 KH domains.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
Abcam recommends using milk as the blocking agent and we expect this antibody to react with KHSRP at approximately 88 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.