Anti-KHSRP抗体(ab95229)
Key features and details
- Rabbit polyclonal to KHSRP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-KHSRP抗体
参阅全部 KHSRP 一抗 -
描述
兔多克隆抗体to KHSRP -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Dog -
免疫原
Synthetic peptide corresponding to Human KHSRP aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107905) -
阳性对照
- This antibody gave a positive signal in the following human whole cell lysates: HeLa; HEK293; MCF7; HepG2.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95229于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 mg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa).
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说明 |
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WB
Use a concentration of 1 mg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 73 kDa). |
靶标
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功能
Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs. -
组织特异性
Detected in neural and non-neural cell lines. -
序列相似性
Belongs to the KHSRP family.
Contains 4 KH domains. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites. - Information by UniProt
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数据库链接
- Entrez Gene: 374140 Chicken
- Entrez Gene: 485022 Dog
- Entrez Gene: 8570 Human
- Entrez Gene: 16549 Mouse
- Entrez Gene: 171137 Rat
- Omim: 603445 Human
- SwissProt: Q92945 Human
- SwissProt: Q3U0V1 Mouse
see all -
别名
- Far upstream element-binding protein 2 antibody
- FBP2 antibody
- FUBP2 antibody
see all
图片
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All lanes : Anti-KHSRP antibody (ab95229) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using milk as the blocking agent and we expect this antibody to react with KHSRP at approximately 88 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab95229 被引用在 1 文献中.
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490