概述

  • 产品名称Anti-Kettin抗体[MAC 155]
  • 描述
    大鼠单克隆抗体[MAC 155] to Kettin
  • 特异性Antibody reacts with 500 and 700 kDa isoforms in Lethocerus and Drosophila flight and non-flight muscle. The epitope is near the middle of the molecule. Antibody reacts with Caenorhabditis (nematode) muscle and does not react with rabbit skeletal muscle.
  • 经测试应用适用于: ICC/IF, Electron Microscopy, WBmore details
  • 种属反应性
    与反应: Caenorhabditis elegans, Drosophila melanogaster, Lethocerus indicus (Waterbug)
    不与反应: Rabbit
  • 免疫原

    Z-discs purified from Lethocerus indicus (Waterbug)

性能

应用

Our Abpromise guarantee covers the use of ab50585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
Electron Microscopy Use at an assay dependent dilution.
WB Use at an assay dependent concentration. PubMed: 22479211

靶标

  • 功能Key component in the assembly and functioning of adult and embryonic striated muscles and muscle tendons. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.
  • 组织特异性Expressed in the mesoderm at stage 11, several hours before myoblast fusion, and persists in most muscle cells, somatic, visceral and pharyngeal muscles and their precursors, until the third instar. Also expressed in adult flight muscle Z-disks.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 7 fibronectin type-III domains.
    Contains 53 Ig-like (immunoglobulin-like) domains.
    Contains 1 SH3 domain.
    Contains 7 TPR repeats.
  • 发展阶段Expressed both maternally and zygotically.
  • 细胞定位Cytoplasm. Nucleus. Chromosome. Uniformly distributed along condensed mitotic chromosomes.
  • Information by UniProt
  • 数据库链接
    • 别名
      • D Titin antibody
      • D-Titin antibody
      • Kettin antibody
      • Sallimus antibody
      • sls antibody
      • Titin antibody
      • TITIN_DROME antibody
      see all

    Anti-Kettin antibody [MAC 155] 图像

    • Immunofluorescence analysis of Drosophila melanogaster larvae muscle, staining Kettin with ab50585.

    Anti-Kettin antibody [MAC 155] (ab50585)参考文献

    This product has been referenced in:
    • Wójtowicz I  et al. Drosophila small heat shock protein CryAB ensures structural integrity of developing muscles, and proper muscle and heart performance. Development 142:994-1005 (2015). Read more (PubMed: 25715399) »
    • Molnár I  et al. DAAM is required for thin filament formation and Sarcomerogenesis during muscle development in Drosophila. PLoS Genet 10:e1004166 (2014). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 24586196) »

    See all 10 Publications for this product

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