Anti-ketohexokinase抗体(ab38281)

概述

  • 产品名称Anti-ketohexokinase抗体
    参阅全部 ketohexokinase 一抗
  • 描述
    兔多克隆抗体to ketohexokinase
  • 经测试应用适用于: IHC-P, ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH selected within the last 50 aa of human ketohexokinase.

  • 阳性对照
    • Mouse kidney tissue lysate. IHC-P: FFPE human kidney tissue sections. IF/ICC: HeLa cell line.

性能

应用

Our Abpromise guarantee covers the use of ab38281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 33 kDa.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 组织特异性Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye.
  • 通路Carbohydrate metabolism; fructose metabolism.
  • 疾病相关Defects in KHK are the cause of fructosuria (FRUCT) [MIM:229800]. Fructosuria is a benign defect of intermediary metabolism.
  • 序列相似性Belongs to the carbohydrate kinase pfkB family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 2.7.1.3 antibody
    • Fructokinase antibody
    • Hepatic fructokinase antibody
    • Ketohexokinase antibody
    • Ketohexokinase isoform a antibody
    • KHK antibody
    • KHK_HUMAN antibody
    see all

Anti-ketohexokinase antibody 图像

  • ICC/IF image of ab38281 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38281, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ketohexokinase staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38281, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-ketohexokinase antibody (ab38281) at 1/100 dilution + Mouse kidney tissue lysate

    Predicted band size : 33 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa. We are unsure as to the identity of these extra bands.

Anti-ketohexokinase antibody (ab38281)参考文献

ab38281 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Archilochus colubris Tissue lysate - whole (pectoralis, ankle flexors and extensors, liver, he)
Gel Running Conditions Reduced Denaturing (10% gel, PVDF membrane)
Loading amount 10 µg
Specification pectoralis, ankle flexors and extensors, liver, he
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Dr. Kenneth Welch

Verified customer

提交于 May 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Chicken Tissue lysate - whole (pectoralis)
Gel Running Conditions Reduced Denaturing (10% gel, PVDF membrane)
Loading amount 10 µg
Specification pectoralis
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Dr. Kenneth Welch

Verified customer

提交于 May 06 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"