概述

  • 产品名称Anti-Keap1抗体
    参阅全部 Keap1 一抗
  • 描述
    山羊多克隆抗体to Keap1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    EVTPSQHGNRTFS

    with a Cysteine residue linker, corresponding to internal sequence amino acids 41-53 of Human Keap1 (NP_036421.2).

  • 阳性对照
    • HeLa and NIH3T3 cell lysates.
  • 常规说明Reported variants represent identical protein: NP_036421.2, NP_987096.1.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab118285 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab118285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • 组织特异性Broadly expressed, with highest levels in skeletal muscle.
  • 序列相似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • 结构域The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • 翻译后修饰Ubiquitinated and subject to proteasomal degradation.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Anti-Keap1 antibody 图像

  • Anti-Keap1 antibody (ab118285) at 0.2 µg/ml + NIH3T3 cell lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 70 kDa
  • ab118285 (3.8µg/ml) staining of paraffin embedded Human Cerebellum following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows textured cytoplasm staining in Purkinje cell and processes.

Anti-Keap1 antibody (ab118285)参考文献

ab118285 has not yet been referenced specifically in any publications.

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