Anti-Keap1抗体[1B4] (ab119403)

概述

  • 产品名称Anti-Keap1抗体[1B4]
    参阅全部 Keap1 一抗
  • 描述
    小鼠单克隆抗体[1B4] to Keap1
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length Human Keap1 produced in HEK293T cells (NP_987096).

  • 阳性对照
    • HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA; HEK293T cells transfected with pCMV6-ENTRY Keap1 overexpress plasmid; Jurkat and HeLa cells; Human endometrium, endometrium adenocarcinoma and bladder carcinoma tissues.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab119403 was purified from Mouse ascites fluid by affinity chromatography.
  • 克隆单克隆
  • 克隆编号1B4
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab119403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 70 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
  • 组织特异性Broadly expressed, with highest levels in skeletal muscle.
  • 序列相似性Contains 1 BACK (BTB/Kelch associated) domain.
    Contains 1 BTB (POZ) domain.
    Contains 6 Kelch repeats.
  • 结构域The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
  • 翻译后修饰Ubiquitinated and subject to proteasomal degradation.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosolic inhibitor of Nrf2 antibody
    • INrf 2 antibody
    • INrf2 antibody
    • Keap 1 antibody
    • KEAP1 antibody
    • KEAP1_HUMAN antibody
    • Kelch like ECH associated protein 1 antibody
    • Kelch like family member 19 antibody
    • Kelch like protein 19 antibody
    • Kelch-like ECH-associated protein 1 antibody
    • Kelch-like protein 19 antibody
    • KIAA0132 antibody
    • KLHL 19 antibody
    • KLHL19 antibody
    • MGC10630 antibody
    • MGC1114 antibody
    • MGC20887 antibody
    • MGC4407 antibody
    • MGC9454 antibody
    see all

Anti-Keap1 antibody [1B4] 图像

  • All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Keap1 cDNA

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 70 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
  • All lanes : Anti-Keap1 antibody [1B4] (ab119403) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : SVT2 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 70 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202189)
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
  • ab119403 at 1/150 dilution staining Keap1 in paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.
  • ab119403 at 1/100 dilution staining Keap1 in HEK293T cells transfected with either pCMV6-ENTRY Keap1 overexpress plasmid (Red) or empty vector control plasmid (Blue) and then analysed by Flow Cytometry.
  • ab119403 at 1/100 dilution staining Keap1 in HeLa cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
  • ab119403 at 1/100 dilution staining Keap1 in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).

Anti-Keap1 antibody [1B4] (ab119403)参考文献

This product has been referenced in:
  • Ziros PG  et al. Nrf2 is commonly activated in papillary thyroid carcinoma, and it controls antioxidant transcriptional responses and viability of cancer cells. J Clin Endocrinol Metab 98:E1422-7 (2013). Read more (PubMed: 23766517) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (prostate)
Specification prostate
Permeabilization Yes - triton
Fixative Formaldehyde
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Verified customer

提交于 Jul 28 2014

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