Anti-KDM6B / JMJD3抗体(ab84200)

概述

  • 产品名称Anti-KDM6B / JMJD3抗体
    参阅全部 KDM6B / JMJD3 一抗
  • 描述
    兔多克隆抗体to KDM6B / JMJD3
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to Human KDM6B/ JMJD3 aa 2-51 (N terminal).
    Sequence:

    HRAVDPPGARAAREAFALGGLSCAGAWSSCPPHPPPRSAWLPGGRCSASI


    Database link: O15054

  • 阳性对照
    • Human fetal lung lysate.

性能

应用

Our Abpromise guarantee covers the use of ab84200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 181 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • 序列相似性Belongs to the UTX family.
    Contains 1 JmjC domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Histone demethylase JMJD3 antibody
    • JmjC domain containing protein 3 antibody
    • JmjC domain-containing protein 3 antibody
    • Jumonji D3 antibody
    • Jumonji domain containing 3 antibody
    • Jumonji domain containing 3 histone lysine demethylase antibody
    • Jumonji domain containing protein 3 antibody
    • Jumonji domain-containing protein 3 antibody
    • Kdm6b antibody
    • KDM6B_HUMAN antibody
    • KIAA0346 antibody
    • Lysine demethylase 6B antibody
    • Lysine K specific demethylase 6B antibody
    • Lysine specific demethylase 6B antibody
    • Lysine-specific demethylase 6B antibody
    see all

Anti-KDM6B / JMJD3 antibody 图像

  • Anti-KDM6B / JMJD3 antibody (ab84200) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 181 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)

Anti-KDM6B / JMJD3 antibody (ab84200)参考文献

ab84200 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (5-15%)
Sample Mouse Cell lysate - whole cell (Human Fibroblasts Mouse Primary hippocampal neuron)
Specification Human Fibroblasts Mouse Primary hippocampal neuron
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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提交于 Sep 27 2013

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Thank you for your reply.
I am sorry to hear that you are struggeling so much with these antibodies.

I have checked again the datasheet for ab85392: Mouse was shown to work in the publication that is listed on the datasheet:

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I am sorry to hear that the KDM6B antibodies do not work for you satisfactorily.

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