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Our Abpromise guarantee covers the use of ab85392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|CLIP||Use at an assay dependent concentration. PubMed: 22589717|
|ChIP||Use at an assay dependent concentration. PubMed: 21095589|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 180 kDa.|
Blocking buffer: 5% non-fat milk in TBST.
Immunocytochemistry/Immunofluorescence: ab85392 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with ab85392 at a 1/200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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