KDM6A/KDM6B Activity Quantification Assay试剂盒(Fluorometric) (ab156911)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Nuclear Extracts, Purified protein
- Sensitivity: 10 ng
概述
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产品名称
KDM6A/KDM6B Activity Quantification Assay试剂盒(Fluorometric)
参阅全部 KDM6A/KDM6B 试剂盒 -
检测方法
Fluorescent -
样品类型
Nuclear Extracts, Purified protein -
检测类型
Enzyme activity -
灵敏度
10 ng -
检测时间
3h 00m -
种属反应性
与反应: Plants, Mammals, Fungi -
产品概述
KDM6A/KDM6B Activity Quantification Assay Kit (Fluorometric) (ab156911) is a complete set of optimized reagents, designed for measuring activity/inhibition of KDM6A and KDM6B using nuclear extracts or purified enzymes from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. There are currently a limited number of methods used for detecting KDM6A/KDM6B activity/inhibition.
The traditional method is based on the measurement of formaldehyde release, a by-product of KDM6A/KDM6B enzymatic reaction, and has significant weaknesses: (1) large amounts (at µg level) of substrate and enzyme are required; (2) nuclear extracts from cell/tissues cannot be used; (3) redox-sensitive KDM6A/KDM6B inhibitiors are not suitable for testing with such methods; (4) high intereference by SDS, DMSO, thiol-containing chemicals, and ions, which are often contained in enzyme solutions, tested compound solvents, and assay buffers; and (5) less accurate than direct measurement of KDM6A/KDM6B-converted demethylated products.
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说明
Lysine specific demethylase 6A (KDM6A/UTX) and lysine specific demethylase 6B (KDM6B/JMJD3) demethylase lysine 27 on histone H3, thereby playing a central role in histone code. KDM6A and KDM6B are JmjC (Jumonji)-domain-containing proteins and catalyze the removal of di- and tri-methylation from histone H3-K27 by using a hydroxylation reaction with iron and alpha-glutarate as cofactors.
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 1 x 6 units 1 x 12 units Adhesive Covering Film 1 unit 1 unit Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 10µl Co-factor 1 1 x 30µl 1 x 60µl Co-factor 2 1 x 30µl 1 x 60µl Co-factor 3 1 x 30µl 1 x 60µl Detection Antibody, 400 µg/mL 1 x 6µl 1 x 12µl Dilution Buffer 1 x 4ml 1 x 8ml Fluoro-Developer 1 x 10µl 1 x 20µl Fluoro-Enhancer 1 x 10µl 1 x 20µl Assay Buffer 1 x 4ml 1 x 8ml Assay Standard, 50 µg/mL 1 x 10µl 1 x 20µl Substrate, 50 µg/mL 1 x 60µl 1 x 120µl -
研究领域
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相关性
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. -
细胞定位
Nuclear -
别名
- bA386N14.2
- Histone demethylase UTX
- JmjC domain containing protein 3
see all
相关产品
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Related Products
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab156911 尚未被引用在任何文献中。