这个 快线抗体 还未被充份测试和鉴定,因此它受制于相关的 使用条款

概述

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 154 kDa.Can be blocked with Human KDM6A / UTX peptide (ab36937).
ICC/IF Use a concentration of 1 µg/ml.
ChIP Use at an assay dependent concentration. PubMed: 26306033

靶标

  • 功能
    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.
  • 序列相似性
    Belongs to the UTX family.
    Contains 1 JmjC domain.
    Contains 8 TPR repeats.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) antibody
    • bA386N14.2 antibody
    • bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX antibody
    • DKFZp686A03225 antibody
    • Histone demethylase UTX antibody
    • KABUK2 antibody
    • Kdm6a antibody
    • KDM6A_HUMAN antibody
    • Lysine (K) specific demethylase 6A antibody
    • Lysine K specific demethylase 6A antibody
    • Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome antibody
    • Lysine-specific demethylase 6A antibody
    • MGC141941 antibody
    • Ubiquitously transcribed tetratricopeptide repeat X chromosome antibody
    • Ubiquitously transcribed tetratricopeptide repeat protein X linked antibody
    • Ubiquitously transcribed TPR gene on the X chromosome antibody
    • Ubiquitously transcribed TPR protein on the X chromosome antibody
    • Ubiquitously transcribed X chromosome tetratricopeptide repeat protein antibody
    • Ubiquitously-transcribed TPR protein on the X chromosome antibody
    • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein antibody
    • UTX antibody
    see all

图片

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/ml

    Lane 1 : Whole NIH3T3 cell lysate (no shRNA)
    Lane 2 : Whole NIH3T3 cell lysate (Control shRNA)
    Lane 3 : Whole NIH3T3 cell lysate (Utx shRNA)

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit polyclonal
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 154 kDa
    Observed band size : 148 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    Lane 3 is NIH3T3 cells transduced with lentivirus expressing shRNA for mouse Utx.

    5% milk was used as the blocking agent (incubated for 1 hour at 25°C).

    ab36938 was diluted with TBS+0.1% Tween 20 and incubated for 1 hour at 25°C. 

    See Abreview

  • All lanes : Anti-KDM6A / UTX antibody (ab36938) at 1 µg/ml

    Lane 1 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
    Lane 2 : Testis (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG -H&L- Pre Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 154 kDa
    Observed band size : 154,157 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.This Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
  • ICC/IF image of ab36938 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab36938, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa cells fixed in 4% PFA at 1ug/ml and HeLa and MCF7 100% methanol fixed cells at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

文献

This product has been referenced in:
  • Xun J  et al. JMJD3 suppresses stem cell-like characteristics in breast cancer cells by downregulation of Oct4 independently of its demethylase activity. Oncotarget 8:21918-21929 (2017). WB ; Human . Read more (PubMed: 28423536) »
  • Li F  et al. Histone Deacetylase 1 (HDAC1) Negatively Regulates Thermogenic Program in Brown Adipocytes via Coordinated Regulation of Histone H3 Lysine 27 (H3K27) Deacetylation and Methylation. J Biol Chem 291:4523-36 (2016). Read more (PubMed: 26733201) »

See all 16 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (7,5 %, tank blot to PVDF)
Sample
Human Cell lysate - whole cell (U2OS cell line)
Specification
U2OS cell line
Treatment
-/+ transfection with full length UTX expression plasmid
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jun 20 2013

Thank you for your reply and for confirming the steps of your protocol.


Since these antibodies are Fast Track antibodies, they are only guaranteed to work in ELISA using the peptide immunogen. These are not tested in-house, and therefor...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain Section)
Specification
Brain Section
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization
Yes - 0.1% Tween20
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 08 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Cell lysate - whole cell (Heart, Liver and Kidney)
Loading amount
25 µg
Specification
Heart, Liver and Kidney
Gel Running Conditions
Reduced Denaturing (NuPAGE BisTris 10% gel (invitrogen))
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0% · Temperature: 22°C
Username

Dr. Vanessa Hall

Verified customer

提交于 Aug 20 2010

Application
Western blot
Sample
Mouse Cell lysate - nuclear (NIH3T3 cell lysate)
Loading amount
20 µg
Specification
NIH3T3 cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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