Anti-KDM6A / UTX抗体[3A2] (ab91231)

概述

  • 产品名称Anti-KDM6A / UTX抗体[3A2]
    参阅全部 KDM6A / UTX 一抗
  • 描述
    小鼠单克隆抗体[3A2] to KDM6A / UTX
  • 经测试应用适用于: Flow Cyt, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Human KDM6A/ UTX.
    Database link: O15550

  • 阳性对照
    • Expressed UTX in 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab91231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 154 kDa.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A.
  • 序列相似性Belongs to the UTX family.
    Contains 1 JmjC domain.
    Contains 8 TPR repeats.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX) antibody
    • bA386N14.2 antibody
    • bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX antibody
    • DKFZp686A03225 antibody
    • Histone demethylase UTX antibody
    • KABUK2 antibody
    • Kdm6a antibody
    • KDM6A_HUMAN antibody
    • Lysine (K) specific demethylase 6A antibody
    • Lysine K specific demethylase 6A antibody
    • Lysine specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat gene X chromosome antibody
    • Lysine-specific demethylase 6A antibody
    • MGC141941 antibody
    • Ubiquitously transcribed tetratricopeptide repeat X chromosome antibody
    • Ubiquitously transcribed tetratricopeptide repeat protein X linked antibody
    • Ubiquitously transcribed TPR gene on the X chromosome antibody
    • Ubiquitously transcribed TPR protein on the X chromosome antibody
    • Ubiquitously transcribed X chromosome tetratricopeptide repeat protein antibody
    • Ubiquitously-transcribed TPR protein on the X chromosome antibody
    • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein antibody
    • UTX antibody
    see all

Anti-KDM6A / UTX antibody [3A2] 图像



  • Predicted band size : 154 kDa
  • Overlay histogram showing MCF7 cells stained with ab91231 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91231, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-KDM6A / UTX antibody [3A2] (ab91231)参考文献

ab91231 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Bladder)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 30 µg
Treatment Lentivirus for gene knock-out
Specification Bladder
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Jinwoo Ahn

Verified customer

提交于 May 20 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step None
Permeabilization Yes - 10% Tween-20
Specification Brain
Blocking step Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 22 2016

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I am sorry to hear that you are having some trouble with the antibody. I am sending you a free positive control lysate of MCF7 cells, ab3871. To check the status of this order, please call Customer Service at 888-...

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I am very pleased to hear you would like to accept our offer and test ab91231 in IHC PFA-fixed. This code will give you: 1 free PRIMARY ANTIBODYbefore the ...

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Thank you for your inquiry.

We have ab91231 which is tested in IP and WB using mouse samples.

http://www.abcam.com/KDM6A-UTX-antibody-3A2-ab91231.html

I hope this information helps. Please contact us with any other questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"