Anti-KDM5A / Jarid1A / RBBP2抗体[18E8] (ab78322)

概述

  • 产品名称Anti-KDM5A / Jarid1A / RBBP2抗体[18E8]
    参阅全部 KDM5A / Jarid1A / RBBP2 一抗
  • 描述
    小鼠单克隆抗体[18E8] to KDM5A / Jarid1A / RBBP2
  • 特异性Specific to human and mouse RBBP2. Can detect endogenous levels of RBBP2.
  • 经测试应用适用于: Flow Cyt, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human KDM5A/ Jarid1A/ RBBP2 aa 1416-1434.
    Sequence:

    PRKQPRKSPLVPRSLEPPV


    Database link: P29375

  • 阳性对照
    • HeLa siRNA. HeLa RBP2 siRNA. Extracts from MCF7 cells, U2OS cells, NIH3T3 cells and J1 (mouse ES) cells.
  • 常规说明

    Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code.

性能

应用

Our Abpromise guarantee covers the use of ab78322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 196 kDa.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12.
  • 序列相似性Belongs to the JARID1 histone demethylase family.
    Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 3 PHD-type zinc fingers.
  • 结构域The GSGFP motif is required for the interaction with SUZ12.
  • 细胞定位Nucleus > nucleolus. Occupies promoters of genes involved in RNA metabolism and mitochondrial function.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Histone demethylase JARID1A antibody
    • JARID1A antibody
    • Jumonji/ARID domain containing protein 1A antibody
    • Jumonji/ARID domain-containing protein 1A antibody
    • Kdm5a antibody
    • KDM5A_HUMAN antibody
    • Lysine-specific demethylase 5A antibody
    • RBBP-2 antibody
    • RBBP2 antibody
    • RBP2 antibody
    • Retinoblastoma binding protein 2 antibody
    • Retinoblastoma-binding protein 2 antibody
    see all

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] 图像

  • All lanes : Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] (ab78322) at 1 µg/ml

    Lane 1 : HeLa control siRNA
    Lane 2 : HeLa RBBP2 siRNA
    Lane 3 : Extracts from MCF7 cells
    Lane 4 : Extracts from U2OS cells
    Lane 5 : Extracts from NIH3T3 cells
    Lane 6 : Extracts from J1 (mouse ES) cells

    Developed using the ECL technique

    Predicted band size : 196 kDa
    Observed band size : 196 kDa
  • ab78322 staining KDM5A / Jarid1A / RBBP2 [18E8] in HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4 % paraformaldehyde, permeabilized with Triton X-100 0.25% in PBS and blocked with 1.5% BSA for 30 minutes. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for overnight at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/1000 in PBS + 1% BSA) was used as the secondary antibody and incubated for 1 hour at room temperature. DAPI was used to stain the nuclear DNA.

  • Overlay histogram showing HeLa cells stained with ab78322 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78322, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-KDM5A / Jarid1A / RBBP2 antibody [18E8] (ab78322)参考文献

This product has been referenced in:
  • Lopez-Bigas N  et al. Genome-wide analysis of the H3K4 histone demethylase RBP2 reveals a transcriptional program controlling differentiation. Mol Cell 31:520-30 (2008). Read more (PubMed: 18722178) »
  • Klose RJ  et al. The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Cell 128:889-900 (2007). Read more (PubMed: 17320163) »

See all 3 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab78322.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"