概述

  • 产品名称Anti-KDM4D / JMJD2D抗体
    参阅全部 KDM4D / JMJD2D 一抗
  • 描述
    兔多克隆抗体to KDM4D / JMJD2D
  • 经测试应用适用于: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-354 of Human KDM4D/ JMJD2D (43kDa).

  • 阳性对照
    • DLD1 (Human colorectal adenocarcinoma) cells; DLD1 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab93694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/200. Predicted molecular weight: 58 kDa.
IP Use at 20 µg/mg of lysate.
Flow Cyt Use a concentration of 5 µg/ml.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • 序列相似性Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • JHD3D antibody
    • JHDM3D antibody
    • JmjC domain-containing histone demethylation protein 3D antibody
    • Jumonji domain-containing protein 2D antibody
    • Kdm4d antibody
    • KDM4D_HUMAN antibody
    • Lysine-specific demethylase 4D antibody
    see all

Anti-KDM4D / JMJD2D antibody 图像

  • ab93694, at 10µg/ml, staining KDM4D / JMJD2D in DLD1 (Human Colorectal Adenocarcinoma) cells by Immunofluorescence. Cells were fixed with 1% formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with normal goat serum. Secondary antibody: Goat anti Rabbit Cy3 at 1/250 dilution.
  • All lanes : Anti-KDM4D / JMJD2D antibody (ab93694) at 1/200 dilution

    Lane 1 : DLD1 cell lysate at 10 µg
    Lane 2 : DLD1 cell lysate at 5 µg


    Predicted band size : 58 kDa
  • IHC image of ab93694 staining in Human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93694, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-KDM4D / JMJD2D antibody (ab93694)参考文献

This product has been referenced in:
  • Badal S  et al. Nonantibiotic Effects of Fluoroquinolones in Mammalian Cells. J Biol Chem 290:22287-97 (2015). WB ; Human . Read more (PubMed: 26205818) »
  • Khoury-Haddad H  et al. PARP1-dependent recruitment of KDM4D histone demethylase to DNA damage sites promotes double-strand break repair. Proc Natl Acad Sci U S A 111:E728-37 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24550317) »

See all 2 Publications for this product

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