Anti-KDM4C / GASC1 / JMJD2C抗体(ab27532)

概述

  • 产品名称
    Anti-KDM4C / GASC1 / JMJD2C抗体
    参阅全部 KDM4C / GASC1 / JMJD2C 一抗
  • 描述
    兔多克隆抗体to KDM4C / GASC1 / JMJD2C
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human GASC1/ JMJD2C.

    (Peptide available as ab30652.)

  • 阳性对照
    • WB: HeLa, Jurkat, A431, HEK293, HepG2, MCF7 and SHSY-5Y whole cell lysates. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab27532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

靶标

  • 功能
    Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
  • 组织特异性
    Overexpressed in several esophageal squamous cell carcinomas (ESCs).
  • 序列相似性
    Belongs to the JHDM3 histone demethylase family.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • 结构域
    The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA146B14.1 antibody
    • GASC 1 protein antibody
    • GASC-1 protein antibody
    • GASC1 antibody
    • Gene amplified in squamous cell carcinoma 1 protein antibody
    • JHDM3C antibody
    • JmjC domain containing histone demethylation protein 3C antibody
    • JmjC domain-containing histone demethylation protein 3C antibody
    • JMJD2C antibody
    • Jumonji domain containing 2C antibody
    • Jumonji domain containing protein 2C antibody
    • Jumonji domain-containing protein 2C antibody
    • Kdm4c antibody
    • KDM4C_HUMAN antibody
    • Lysine (K) specific demethylase 4C antibody
    • Lysine demethylase 4C antibody
    • Lysine-specific demethylase 4C antibody
    • TDRD14C antibody
    • Tudor domain containing 14C antibody
    see all

图片

  • All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody (ab27532) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 120 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ICC/IF image of ab27532 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27532 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

文献

This product has been referenced in:
  • Lizcano F  et al. Regulation of adipogenesis by nuclear receptor PPAR? is modulated by the histone demethylase JMJD2C. Genet Mol Biol 34:19-24 (2011). WB ; Mouse . Read more (PubMed: 21637537) »

See 1 Publication for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 21 2017


I am very pleased to hear you would like to accept our offer and test these antibodies noted above in IP. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please sub...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
400 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Mr. Antibody Solutions

Verified customer

提交于 May 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, HeLa, Jurkat, U2OS)
Loading amount
20 µg
Specification
HEK293, HeLa, Jurkat, U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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