产品名称 Anti-KDM4B / JMJD2B抗体 参阅全部 KDM4B / JMJD2B 一抗
兔多克隆抗体to KDM4B / JMJD2B
WB, IHC-P more details
Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 10-40 of Human KDM4B/ JMJD2B
K562 cell lysate, Human Testis tissue
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液 Preservative: 0.09% Sodium Azide
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 This antibody is purified through a protein A column, followed by peptide affinity purification.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 122 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
功能 Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Only able to demethylate trimethylated H3 'Lys-9', with a weaker activity than KDM4A, KDM4C and KDM4D. Demethylation of Lys residue generates formaldehyde and succinate.
序列相似性 Belongs to the JHDM3 histone demethylase family. Contains 1 JmjC domain. Contains 1 JmjN domain. Contains 2 PHD-type zinc fingers. Contains 2 Tudor domains.
结构域 The 2 Tudor domains recognize and bind methylated histones. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails.
Information by UniProt
JmjC domain-containing histone demethylation protein 3B antibody
Anti-KDM4B / JMJD2B antibody 图像
Western blot - KDM4B / JMJD2B antibody (ab103129)
Anti-KDM4B / JMJD2B antibody (ab103129) at 1/100 dilution + K562 cell lysate at 35 µg Predicted band size : 122 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KDM4B / JMJD2B antibody (ab103129)
Formalin-fixed and paraffin-embedded Human Testis tissue, which was peroxidase-conjugated to the
secondary antibody, followed by DAB staining, using ab103129 at a dilution of 1/50.
Anti-KDM4B / JMJD2B antibody (ab103129)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"