Anti-KDM4A / JHDM3A / JMJD2A抗体(ab70786)

概述

  • 产品名称Anti-KDM4A / JHDM3A / JMJD2A抗体
    参阅全部 KDM4A / JHDM3A / JMJD2A 一抗
  • 描述
    兔多克隆抗体to KDM4A / JHDM3A / JMJD2A
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis , Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1064) of human KDM4A/ JHDM3A/ JMJD2A. (NP_055478.1)

  • 阳性对照
    • Nuclear extract from 293T cells. Whole cell lysates from HeLa and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab70786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 121 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
      Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
    • 组织特异性Ubiquitous.
    • 序列相似性Belongs to the JHDM3 histone demethylase family.
      Contains 1 C2HC pre-PHD-type zinc finger.
      Contains 1 JmjC domain.
      Contains 1 JmjN domain.
      Contains 2 PHD-type zinc fingers.
      Contains 2 Tudor domains.
    • 结构域The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1.
    • 翻译后修饰Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • JHDM3A antibody
      • JmjC domain containing histone demethylation protein 3A antibody
      • JmjC domain-containing histone demethylation protein 3A antibody
      • JMJD2 antibody
      • JMJD2A antibody
      • jumonji C domain containing histone demethylase 3A antibody
      • Jumonji domain containing 2 antibody
      • Jumonji domain containing 2A antibody
      • Jumonji domain containing protein 2A antibody
      • Jumonji domain-containing protein 2A antibody
      • KDM4A antibody
      • KDM4A_HUMAN antibody
      • KIAA0677 antibody
      • Lysine (K) specific demethylase 4A antibody
      • Lysine-specific demethylase 4A antibody
      • TDRD14A antibody
      • Tudor domain containing 14A antibody
      see all

    Anti-KDM4A / JHDM3A / JMJD2A antibody 图像

    • All lanes : Anti-KDM4A / JHDM3A / JMJD2A antibody (ab70786) at 0.04 µg/ml

      Lane 1 : Nuclear extract from 293T cells at 50 µg
      Lane 2 : Nuclear extract from 293T cells at 15 µg
      Lane 3 : Nuclear extract from 293T cells at 5 µg
      Lane 4 : Whole cell lysate from HeLa cells at 50 µg
      Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


      Predicted band size : 121 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human KDM4A / JHDM3A / JMJD2A by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70786 at 3 µg/mg lysate (Lane 1). Lane 2 represents IgG IP control. Subsequent Western blot detection of KDM4A / JHDM3A / JMJD2A was performed using ab70786 at 1 µg/ml.

    Anti-KDM4A / JHDM3A / JMJD2A antibody (ab70786)参考文献

    This product has been referenced in:
    • Koshibu K  et al. Protein phosphatase 1 regulates the histone code for long-term memory. J Neurosci 29:13079-89 (2009). WB ; Mouse . Read more (PubMed: 19828821) »

    See 1 Publication for this product

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