Anti-KDM4A / JHDM3A / JMJD2A抗体[3559D5a] (ab54388)

概述

  • 产品名称Anti-KDM4A / JHDM3A / JMJD2A抗体[3559D5a]
    参阅全部 KDM4A / JHDM3A / JMJD2A 一抗
  • 描述
    小鼠单克隆抗体[3559D5a] to KDM4A / JHDM3A / JMJD2A
  • 经测试应用适用于: WB, Dot blotmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Recombinant fragment

  • 常规说明ab54388 was filtered through a 0.22 µm membrane.

性能

应用

Our Abpromise guarantee covers the use of ab54388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 121 kDa).
Dot blot Use at an assay dependent dilution.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
    Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the JHDM3 histone demethylase family.
    Contains 1 C2HC pre-PHD-type zinc finger.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
    Contains 2 PHD-type zinc fingers.
    Contains 2 Tudor domains.
  • 结构域The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue (H3K4me). Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' (H3K4me3) is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are also able to bind trimethylated histone H3 'Lys-9' (H3K9me3), di- and trimethylated H4 'Lys-20' (H4K20me2 and H4K20me3). Has high affinity for H4K20me2, blocking recruitment of proteins such as TP53BP1.
  • 翻译后修饰Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • JHDM3A antibody
    • JmjC domain containing histone demethylation protein 3A antibody
    • JmjC domain-containing histone demethylation protein 3A antibody
    • JMJD2 antibody
    • JMJD2A antibody
    • jumonji C domain containing histone demethylase 3A antibody
    • Jumonji domain containing 2 antibody
    • Jumonji domain containing 2A antibody
    • Jumonji domain containing protein 2A antibody
    • Jumonji domain-containing protein 2A antibody
    • KDM4A antibody
    • KDM4A_HUMAN antibody
    • KIAA0677 antibody
    • Lysine (K) specific demethylase 4A antibody
    • Lysine-specific demethylase 4A antibody
    • TDRD14A antibody
    • Tudor domain containing 14A antibody
    see all

Anti-KDM4A / JHDM3A / JMJD2A antibody [3559D5a] 图像

Anti-KDM4A / JHDM3A / JMJD2A antibody [3559D5a] (ab54388)参考文献

ab54388 has not yet been referenced specifically in any publications.

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