概述

  • 产品名称Anti-KDM3B / JMJD1B抗体
    参阅全部 KDM3B / JMJD1B 一抗
  • 描述
    兔多克隆抗体to KDM3B / JMJD1B
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
    Database link: Q7LBC6

  • 阳性对照
    • HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab70797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IF 1/50 - 1/250.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
  • 组织特异性Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
  • 序列相似性Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • 结构域Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5qNCA antibody
    • C5orf7 antibody
    • JHDM2B antibody
    • JmjC domain containing histone demethylation protein 2B antibody
    • JmjC domain-containing histone demethylation protein 2B antibody
    • jmjd1b antibody
    • Jumonji domain containing 1B antibody
    • Jumonji domain containing protein 1B antibody
    • Jumonji domain-containing protein 1B antibody
    • KDM3B antibody
    • KDM3B_HUMAN antibody
    • KIAA1082 antibody
    • Lysine (K) specific demethylase 3B antibody
    • Lysine-specific demethylase 3B antibody
    • NET22 antibody
    • Nuclear protein 5qNCA antibody
    see all

Anti-KDM3B / JMJD1B antibody 图像

  • All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 191 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,30 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB.
  • Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
    ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-KDM3B / JMJD1B antibody (ab70797)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions Reduced Denaturing (6% Gel)
Loading amount 500000 cells
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 01 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"