概述

  • 产品名称Anti-KDM3B / JMJD1B抗体
    参阅全部 KDM3B / JMJD1B 一抗
  • 描述
    兔多克隆抗体to KDM3B / JMJD1B
  • 经测试应用适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • 免疫原

    Synthetic peptide corresponding to Human KDM3B/ JMJD1B (C terminal).
    Database link: Q7LBC6

  • 阳性对照
    • HeLa and 293T whole cell lysates. IHC Controls: Laryngeal squamous cell carcinoma, Ovarian carcinoma, Prostate carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab70797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IF 1/50 - 1/250.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 191 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May have tumor suppressor activity.
  • 组织特异性Ubiquitous. Highly expressed in placenta, skeletal muscle, kidney, heart and liver.
  • 序列相似性Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • 结构域Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5qNCA antibody
    • C5orf7 antibody
    • JHDM2B antibody
    • JmjC domain containing histone demethylation protein 2B antibody
    • JmjC domain-containing histone demethylation protein 2B antibody
    • jmjd1b antibody
    • Jumonji domain containing 1B antibody
    • Jumonji domain containing protein 1B antibody
    • Jumonji domain-containing protein 1B antibody
    • KDM3B antibody
    • KDM3B_HUMAN antibody
    • KIAA1082 antibody
    • Lysine (K) specific demethylase 3B antibody
    • Lysine-specific demethylase 3B antibody
    • NET22 antibody
    • Nuclear protein 5qNCA antibody
    see all

Anti-KDM3B / JMJD1B antibody 图像

  • All lanes : Anti-KDM3B / JMJD1B antibody (ab70797) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 191 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,30 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KDM3B / JMJD1B with ab70797 at 1/200 (1µg/ml). Detection: DAB.
  • Detection of KDM3B / JMJD1B by Western Blot of Immunoprecipitate.
    ab70797 at 1µg/ml staining KDM3B / JMJD1B in HeLa whole cell lysate immunoprecipitated using ab70797 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Anti-KDM3B / JMJD1B antibody (ab70797)参考文献

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See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions Reduced Denaturing (6% Gel)
Loading amount 500000 cells
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Nov 01 2016

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