Anti-KDM3A / JHDM2A抗体[14F8] - ChIP Grade (ab91252)

概述

  • 产品名称Anti-KDM3A / JHDM2A抗体[14F8] - ChIP Grade
    参阅全部 KDM3A / JHDM2A 一抗
  • 描述
    小鼠单克隆抗体[14F8] to KDM3A / JHDM2A - ChIP Grade
  • 经测试应用适用于: ChIP, Flow Cyt, IHC-P, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment (Human)

  • 阳性对照
    • Expressed KDM3A in 293T cells. Hela nuclear extracts for IP. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal spleen.

性能

应用

Our Abpromise guarantee covers the use of ab91252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 23386205
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 147 kDa.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

靶标

  • 功能Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
  • 序列相似性Belongs to the JHDM2 histone demethylase family.
    Contains 1 JmjC domain.
  • 结构域The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
    Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors.
  • 细胞定位Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp686A24246 antibody
    • DKFZp686P07111 antibody
    • JHDM2A antibody
    • JHMD2A antibody
    • JmjC domain-containing histone demethylation protein 2A antibody
    • JMJD1 antibody
    • JMJD1A antibody
    • Jumonji C domain containing histone demethylase 2A antibody
    • Jumonji domain containing 1 antibody
    • Jumonji domain containing 1A antibody
    • Jumonji domain containing protein 1A antibody
    • Jumonji domain-containing protein 1A antibody
    • Kdm3a antibody
    • KDM3A_HUMAN antibody
    • KIAA0742 antibody
    • Lysine (K) specific demethylase 3A antibody
    • Lysine-specific demethylase 3A antibody
    • Testis specific protein A antibody
    • TSGA antibody
    see all

Anti-KDM3A / JHDM2A antibody [14F8] - ChIP Grade 图像

  • IHC image of KDM3A / JHDM2A antibody staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91252, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 147 kDa
    Cell extracts derived from 293T without (-) or with overexpressing Flag-KDM3A (+) were used for Western (left panel) and IP-Western (right panel) analysis of ab91252 (clone 14F8).
  • Regular IP buffer: 1mM EDTA; 150mM NaCl; 50mM TrisCl, pH 7.5; 1% Triton X-100; 2mM PMSF; Protease inhibitors ChIP C buffer: 1mM EDTA; 150mM NaCl; 50mM TrisCl, pH 7.5; 1% Triton X-100; 0.1% Sodium Deoxycholate; 2mM PMSF; Protease inhibitors
  • Overlay histogram showing HeLa cells stained with ab91252 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab91252, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-KDM3A / JHDM2A antibody [14F8] - ChIP Grade (ab91252)参考文献

This product has been referenced in:
  • Miao F  et al. RNA-sequencing analysis of high glucose-treated monocytes reveals novel transcriptome signatures and associated epigenetic profiles. Physiol Genomics 45:287-99 (2013). ChIP ; Human . Read more (PubMed: 23386205) »

See 1 Publication for this product

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DISCOUNT CODE: *************** Expiration date: ************ I am very pleased to hear you would like to accept our offer and test ab66654 in rat and mouse tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem th...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Trophoblast stem cell)
Loading amount 100 µg
Specification Trophoblast stem cell
Treatment 0.5% oxygen for 24 hours
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Aug 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"