Anti-KDM2B抗体(ab85807)

概述

  • 产品名称Anti-KDM2B抗体
    参阅全部 KDM2B 一抗
  • 描述
    山羊多克隆抗体to KDM2B
  • 特异性ab85807 is expected to recognize isoform a (NP_115979.3) only.
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Horse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Human KDM2B aa 1324-1336 (C terminal) (Cysteine residue).
    Sequence:

    FGQVEEKLLQKLS


    Database link: Q8NHM5

  • 阳性对照
    • Skin tissue.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab85807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/32000.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • 序列相似性Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • 结构域The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.
  • 细胞定位Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Histone-H3]-lysine-36 demethylase 1B antibody
    • CXXC-type zinc finger protein 2 antibody
    • CXXC2 antibody
    • F box and leucine rich repeat protein 10 antibody
    • F box protein FBL10 antibody
    • F-box and leucine-rich repeat protein 10 antibody
    • F-box protein FBL10 antibody
    • F-box/LRR-repeat protein 10 antibody
    • Fbl10 antibody
    • FBXL10 antibody
    • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
    • JHDM1B antibody
    • JmjC domain-containing histone demethylation protein 1B antibody
    • Jumonji C domain containing histone demethylase 1B antibody
    • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
    • kdm2b antibody
    • KDM2B_HUMAN antibody
    • Lysine (K) specific demethylase 2B antibody
    • Lysine-specific demethylase 2B antibody
    • PCCX2 antibody
    • Protein containing CXXC domain 2 antibody
    • Protein JEMMA antibody
    • Protein-containing CXXC domain 2 antibody
    see all

Anti-KDM2B antibody 图像

  • ab85807 at 5µg/ml staining KDM2B in skin tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded section).

Anti-KDM2B antibody (ab85807)参考文献

ab85807 has not yet been referenced specifically in any publications.

Product Wall

While we have not yet tested this product in that application I was hoping that I might help you to achieve signal with this product. In reviewing your protocol I noticed that paraformaldehyde was used to fix your tissue. Because of the nature of this ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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提交于 Feb 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"