概述

  • 产品名称
  • 描述
    兔多克隆抗体to KDM2B
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human KDM2B aa 1-100 conjugated to keyhole limpet haemocyanin.
    Database link: Q8NHM5
    (Peptide available as ab167447)

  • 阳性对照
    • This antibody gave a positive signal in both HeLa whole cell lysate and HeLa Nuclear lysate.

性能

应用

Our Abpromise guarantee covers the use of ab108276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 152 kDa).

靶标

  • 功能
    Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
  • 序列相似性
    Belongs to the JHDM1 histone demethylase family.
    Contains 1 CXXC-type zinc finger.
    Contains 1 F-box domain.
    Contains 1 JmjC domain.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 PHD-type zinc finger.
  • 结构域
    The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes.
  • 细胞定位
    Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [Histone-H3]-lysine-36 demethylase 1B antibody
    • CXXC-type zinc finger protein 2 antibody
    • CXXC2 antibody
    • F box and leucine rich repeat protein 10 antibody
    • F box protein FBL10 antibody
    • F-box and leucine-rich repeat protein 10 antibody
    • F-box protein FBL10 antibody
    • F-box/LRR-repeat protein 10 antibody
    • Fbl10 antibody
    • FBXL10 antibody
    • JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody
    • JHDM1B antibody
    • JmjC domain-containing histone demethylation protein 1B antibody
    • Jumonji C domain containing histone demethylase 1B antibody
    • Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody
    • kdm2b antibody
    • KDM2B_HUMAN antibody
    • Lysine (K) specific demethylase 2B antibody
    • Lysine-specific demethylase 2B antibody
    • PCCX2 antibody
    • Protein containing CXXC domain 2 antibody
    • Protein JEMMA antibody
    • Protein-containing CXXC domain 2 antibody
    see all

图片

  • All lanes : Anti-KDM2B antibody (ab108276) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 152 kDa
    Observed band size : 165 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 135 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

实验方案

文献

This product has been referenced in:
  • Jiang Y  et al. Local generation of fumarate promotes DNA repair through inhibition of histone H3 demethylation. Nat Cell Biol 17:1158-68 (2015). WB ; Human . Read more (PubMed: 26237645) »
  • Lu S  et al. The transcription factor c-Fos coordinates with histone lysine-specific demethylase 2A to activate the expression of cyclooxygenase-2. Oncotarget 6:34704-17 (2015). WB . Read more (PubMed: 26430963) »

See all 2 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

提交于 May 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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