Anti-KDM1 / LSD1抗体[EPR6826] (ab125017)


  • 产品名称Anti-KDM1 / LSD1抗体[EPR6826]
    参阅全部 KDM1 / LSD1 一抗
  • 描述
    兔单克隆抗体[EPR6826] to KDM1 / LSD1
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human KDM1/ LSD1.

  • 阳性对照
    • T47D, Jurkat, HeLa, 293T, JAR, SW480, PC3, F9, C6, and PC12 cell lysates; JAR cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab125017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    • 功能Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
    • 组织特异性Ubiquitously expressed.
    • 序列相似性Belongs to the flavin monoamine oxidase family.
      Contains 1 SWIRM domain.
    • 结构域The SWIRM domain may act as an anchor site for a histone tail.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Amine oxidase (flavin containing) domain 2 antibody
      • Amine oxidase, flavin containing, 2 antibody
      • AOF2 antibody
      • BHC110 antibody
      • BRAF35 HDAC complex protein BHC110 antibody
      • BRAF35-HDAC complex protein BHC110 antibody
      • BRAF35/HDAC complex, 110-kD subunit antibody
      • CPRF antibody
      • EC1 antibody
      • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
      • Flavin-containing amine oxidase domain-containing protein 2 antibody
      • KDM 1 antibody
      • KDM1 antibody
      • Kdm1a antibody
      • KDM1A_HUMAN antibody
      • KIAA0601 antibody
      • LSD 1 antibody
      • LSD1 antibody
      • Lysine (K) specific demethylase 1 antibody
      • Lysine (K) specific demethylase 1A antibody
      • Lysine demethylase 1A antibody
      • Lysine specific histone demethylase 1 antibody
      • Lysine specific histone demethylase 1A antibody
      • Lysine-specific demethylase 1 antibody
      • Lysine-specific demethylase 1A antibody
      • Lysine-specific histone demethylase 1A antibody
      see all

    Anti-KDM1 / LSD1 antibody [EPR6826] 图像

    • All lanes : Anti-KDM1 / LSD1 antibody [EPR6826] (ab125017) at 1/1000 dilution

      Lane 1 : T47D lysate
      Lane 2 : Jurkat lysate
      Lane 3 : HeLa lysate
      Lane 4 : 293T lysate
      Lane 5 : JAR lysate
      Lane 6 : SW480 lysate
      Lane 7 : PC3 lysate
      Lane 8 : F9 lysate
      Lane 9 : C6 lysate
      Lane 10 : PC12 lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 92 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • ab125017 at 1/50 dilution staining KDM1 / LSD1 in permeabilized JAR cells by Flow cytometry (red) or a rabbit IgG negative (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-KDM1 / LSD1 antibody [EPR6826] (ab125017)参考文献

    ab125017 has not yet been referenced specifically in any publications.

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