Anti-KDM1 / LSD1抗体- ChIP Grade (ab17721)

概述

  • 产品名称Anti-KDM1 / LSD1抗体- ChIP Grade
    参阅全部 KDM1 / LSD1 一抗
  • 描述
    兔多克隆抗体to KDM1 / LSD1 - ChIP Grade
  • 经测试应用适用于: ChIP, IHC-P, IP, ICC/IF, WB, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Human LSD1.

    (Peptide available as ab17763.)

  • 阳性对照
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)

性能

应用

Our Abpromise guarantee covers the use of ab17721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 93 kDa).Can be blocked with Human KDM1 / LSD1 peptide (ab17763).
IHC-Fr Use at an assay dependent concentration. PubMed: 23911933

靶标

  • 功能Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • 结构域The SWIRM domain may act as an anchor site for a histone tail.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase, flavin containing, 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • BRAF35/HDAC complex, 110-kD subunit antibody
    • CPRF antibody
    • EC1 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific demethylase 1 antibody
    • Lysine-specific demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

Anti-KDM1 / LSD1 antibody - ChIP Grade 图像

  • ICC/IF image of ab17721 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab17721, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • All lanes : Anti-KDM1 / LSD1 antibody - ChIP Grade (ab17721) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : NIH 3T3 whole cell lysate (ab7179)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 93 kDa
  • ab17721 staining LSD1 in the mouse c2c12 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200) for 1 hour at 25°C. A diluted (1/2000) Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

  •      ab17721 at a 1/100 dilution in cross-linking ChIP (using formaldehyde) with mouse liver tissue lysate. The protocol from Nguyen, T. T, et al. (2005) Mol. Cell. Biol. 25: 2147-2157 was followed. Semiquantitative PCR was used as the detection step.

    Lane 1: Input

    Lane 2: ab17721

    Lane 3: IgG

    Lane 4: No ab

    This image is courtesy of an Abreview by  Michelle Barton.

    See Abreview

  • Human 293T cells fixed in 4% paraformaldehyde were immunostained with ab17721 (1/200) for LSD1 and detected using FITC labelled anti-rabbit (Green). Nuclear DNA is stained blue with DAPI.

Anti-KDM1 / LSD1 antibody - ChIP Grade (ab17721)参考文献

This product has been referenced in:
  • Petell CJ  et al. An epigenetic switch regulates de novo DNA methylation at a subset of pluripotency gene enhancers during embryonic stem cell differentiation. Nucleic Acids Res 44:7605-17 (2016). WB, IP ; Mouse . Read more (PubMed: 27179026) »
  • Joo YA  et al. Skin Barrier Recovery by Protease-Activated Receptor-2 Antagonist Lobaric Acid. Biomol Ther (Seoul) 24:529-35 (2016). Read more (PubMed: 27169822) »

See all 53 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 15 µg
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 25 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - Brij
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (lung)
Loading amount 50 µg
Specification lung
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Ms. JIHUN LEE

Verified customer

提交于 Jan 10 2013

Thank you for your message and for kindly providing these details.

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