使用敲除细胞株进行验证

Anti-KDM1 / LSD1抗体[1B2F2] (ab69535)

概述

  • 产品名称Anti-KDM1 / LSD1抗体[1B2F2]
    参阅全部 KDM1 / LSD1 一抗
  • 描述
    小鼠单克隆抗体[1B2F2] to KDM1 / LSD1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human LSD1 truncated protein

  • 阳性对照
    • HeLa cell extract NIH/3T3 cell extract
  • 常规说明Concentration: See product label

性能

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应用

Our Abpromise guarantee covers the use of ab69535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa).

靶标

  • 功能Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • 结构域The SWIRM domain may act as an anchor site for a histone tail.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase, flavin containing, 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • BRAF35/HDAC complex, 110-kD subunit antibody
    • CPRF antibody
    • EC1 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific demethylase 1 antibody
    • Lysine-specific demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

Anti-KDM1 / LSD1 antibody [1B2F2] 图像



  • Predicted band size : 93 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: KDM1 / LSD1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab69535 observed at 110 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab69535 was shown to specifically react with KDM1 / LSD1 when KDM1 / LSD1 knockout samples were used. Wild-type and KDM1 / LSD1 knockout samples were subjected to SDS-PAGE. ab69535 and ab181602 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-KDM1 / LSD1 antibody [1B2F2] (ab69535) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : HeLa cell extract
    Lane 3 : NIH/3T3 cell extract

    Developed using the ECL technique

    Predicted band size : 93 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 112 kDa. We are unsure as to the identity of these extra bands.

Anti-KDM1 / LSD1 antibody [1B2F2] (ab69535)参考文献

ab69535 has not yet been referenced specifically in any publications.

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