使用敲除细胞株进行验证

Anti-KDM1 / LSD1抗体[LSD1-12] (ab53269)

概述

  • 产品名称
    Anti-KDM1 / LSD1抗体[LSD1-12]
    参阅全部 KDM1 / LSD1 一抗
  • 描述
    小鼠单克隆抗体[LSD1-12] to KDM1 / LSD1
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide:

    AMYTLPRQATPGVPAQ

    , corresponding to amino acids 832-847 of Human LSD1

  • 阳性对照
    • ICC/IF: HAP1-KDM1A cells.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab129195 as a replacement.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    LSD1-12
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa).

靶标

  • 功能
    Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis.
  • 组织特异性
    Ubiquitously expressed.
  • 序列相似性
    Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
  • 结构域
    The SWIRM domain may act as an anchor site for a histone tail.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Amine oxidase (flavin containing) domain 2 antibody
    • Amine oxidase, flavin containing, 2 antibody
    • AOF2 antibody
    • BHC110 antibody
    • BRAF35 HDAC complex protein BHC110 antibody
    • BRAF35-HDAC complex protein BHC110 antibody
    • BRAF35/HDAC complex, 110-kD subunit antibody
    • CPRF antibody
    • EC1 antibody
    • FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody
    • Flavin-containing amine oxidase domain-containing protein 2 antibody
    • KDM 1 antibody
    • KDM1 antibody
    • Kdm1a antibody
    • KDM1A_HUMAN antibody
    • KIAA0601 antibody
    • LSD 1 antibody
    • LSD1 antibody
    • Lysine (K) specific demethylase 1 antibody
    • Lysine (K) specific demethylase 1A antibody
    • Lysine demethylase 1A antibody
    • Lysine specific histone demethylase 1 antibody
    • Lysine specific histone demethylase 1A antibody
    • Lysine-specific demethylase 1 antibody
    • Lysine-specific demethylase 1A antibody
    • Lysine-specific histone demethylase 1A antibody
    see all

图片

  • Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: KDM1A knockout HAP1 whole cell lysate (40 µg)
    Lane 3: HeLa whole cell lysate (40 µg)
    Lane 4: Jurkat whole cell lysate (40 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab53269 observed at 92 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab53269 was shown to specifically react with KDM1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KDM1 knockout samples were used. Wild-type and KDM1 knockout samples were subjected to SDS-PAGE.  Ab53269 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2 µg/ml concentration and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • ab53269 staining KDM1A in wild-type HAP1 cells (top panel) and KDM1A knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab53269 at 1μg/ml concentration and ab202272 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

文献

ab53269 has not yet been referenced specifically in any publications.

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