This monoclonal antibody reacts with the carboxy-terminal KDEL motif responsible for the endoplasmic reticulum (ER) localisation of several proteins. It is applicable as an ER marker in a wide variety of species (insects, mammals, plants) and also marks the ER-derived ‘ricinosome’ compartment in senescing plant tissue. It reacts strongly with protein
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/300. PubMed: 20392701
Use at an assay dependent dilution.
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins (GRP 78) and GRP 94 respectively and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
Lys Asp Glu Leu antibody
Immunocytochemistry/ Immunofluorescence - Anti-KDEL antibody [MAC 256] (ab50601)Image from Veiga-da-Cunha M et., at. J Biol Chem. 2010 Jun 11;285(24):18888-98. (Fig 5a)
ab50601 at 1/300 dilution staining KDEL-ER Marker in Chinese Hamster Ovary cells by Immunocytochemistry/ Immunofluorescence. Cells were seeded in 24-well plates on glass coverslips coated with 25 μg/μl fibronectin. An ALexa Fluor labelled anti rat secondary was used at 5µg/ml.