Anti-KCTD18抗体(ab33709)

概述

  • 产品名称
    Anti-KCTD18抗体
  • 描述
    兔多克隆抗体to KCTD18
  • 特异性
    ab33709 recognises KCTD18.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    A region within synthetic peptide: YLNTSGASCE SRIIGVYATK TDGTDAIEKQ LGGRIHSKGI FKREAGNNVQ, corresponding to internal sequence amino acids 147-196 of Human KCTD18

  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab33709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-KCTD18 antibody (ab33709) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at a dilution of 1/50000 - 1/100000

    Predicted band size : 47 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab33709 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
20 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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提交于 Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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