Anti-KCTD18抗体(ab33709)

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概述

  • 产品名称
    Anti-KCTD18抗体
  • 描述
    兔多克隆抗体to KCTD18
  • 宿主
    Rabbit
  • 特异性
    ab33709 recognises KCTD18.
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    A region within synthetic peptide: YLNTSGASCE SRIIGVYATK TDGTDAIEKQ LGGRIHSKGI FKREAGNNVQ, corresponding to internal sequence amino acids 147-196 of Human KCTD18

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Jurkat cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab33709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/312500.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Western blot - Anti-KCTD18 antibody (ab33709)
    Western blot - Anti-KCTD18 antibody (ab33709)
    Anti-KCTD18 antibody (ab33709) at 5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti Rabbit IgG at a dilution of 1/50000 - 1/100000

    Predicted band size: 47 kDa
    Observed band size: 42 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab33709 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab33709？请让我们知道，以便我们可以引用本数据表中的参考文章。

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Western blot abreview for Anti-KCTD18 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
20 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Dec 10 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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